Jeffrey D. Hardin, Ph.D.

Professor
Department of Zoology
jdhardin@wisc.edu

Professor and Director, Biology Core Curriculum

Trainer in the Following Programs:

  • Cellular and Molecular Biology
  • Genetics, Molecular and Cellular Pharmacology
  • Cellular and Molecular Pathology
  • Biophysics
  • Zoology

Honors and Awards:

  • Not Available

Research Description:

We study how sheets of cells (epithelial cells) rearrange, migrate, and adhere to one another in the forming epidermis, or hypodermis, of the early C. elegans embryo. We use a variety of approaches to study epithelial morphogenesis, including genetics, genomics, and advanced microscopy. Because the events we study occur in all animal embryos, what we are discovering has relevance for understanding fundamental processes during normal embryonic development. By studying what molecular processes control cell movements and cell adhesion, we hope to shed light on basic mechanisms of cancer metastasis, and on the events that lead to common birth defects.

C. elegans embryo undergoing ventral enclosure expressing a Pdlg-1::gfp transcriptional reporter pseudocolored cyan, imaged using a Nikon 1.45 NA TIRF objective on our Perkin-Elmer UltraView spinning disk confocal microscope [Mark Sheffield].

 

Selected Publications: Articles on PubMed

  • Lockwood C, Lynch A, and Hardin J. (2008). Dynamic analysis identifies novel roles for DLG-1 subdomains in AJM-1 recruitment and LET-413 dependent apical focusing. J Cell Sci. 121:1477-1487 Article

  • Hardin J, King R, Thomas-Virnig C, and Raich WB. (2008). Zygotic loss of ZEN-4/MKLP1 results in disruption of epidermal morphogenesis in the C. elegans embryo. Dev Dyn. 237:830-6. Article

  • Stevenson TO, Mercer K, Cox EA, Szewczyk NJ, Conley CA, Hardin JD, and Benian GM. (2007). unc-94 encodes a tropomodulin in C. elegans. J Mol Biol. 374:936-50. Article

  • Sheffield M, Loveless T, Hardin J, and Pettitt J. (2007). C. elegans Enabled Exhibits Novel Interactions with N-WASP, Abl, and Cell-Cell Junctions. Curr Biol. Epub ahead of print. PMID 17935994

  • Batchelder EL, Thomas-Virnig CL, Hardin JD, and White JG. (2007). Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo. FEBS Lett. 581:4337-41. PMID 17716666

  • Sims PA and Hardin JD. (2007). Fluorescence-integrated transmission electron microscopy images: integrating fluorescence microscopy with transmission electron microscopy. Methods Mol Biol. 369:291-308. PMID 17656756

  • Phillips BT, Kidd AR 3rd, King R, Hardin J, and Kimble J. (2007). Reciprocal asymmetry of SYS-1/beta-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans. Proc Natl Acad Sci USA. 104:3231-3236. PMID 17296929

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