Eukaryotic Molecular Biology
Pharmacology 620/Biochemistry 620

 

Dr, David Wassarman, 371 Medical Sciences Center / 262-6648 / dawassarman@wisc.edu
Dr. Aseem Ansari, 415C Biochemistry Addition / 265-4690 / ansari@biochem.wisc.edu
Philipp Raess, 563 biochemistry Addition / 262-4705 ex.2546/ pwraess@wisc.edu

Wednesday/Friday 11:00-11:50, Room 108 Plant Sciences


 
Session
number
Date Lecture topic Reading-Weaver and papers Homework
questions/
answers
Instructor

1

Jan 21 Orientation-course outline and goals   Sess. 1 Wassarman/Ansari
2 Jan 23 Overview of gene expression mechanisms-Central dogma Chapters 1-3, 1-57 Sess. 2 Wassarman
3 Jan 28 RNA pol I and pol III transcription Chapter 11, 325-338, Moss, Paule, Schramm Sess. 3 Wassarman
4 Jan 30 RNA polymerases and gene promoters Chapter 10, 261-294, Butler, Asturias Sess. 4 Ansari
5 Feb 4 RNA pol II transcription-general transcription factors Chapter 11, 297-324, Malik, Hochheimer, Tjian, Roeder Sess. 5 Ansari
6 Feb 6 RNA pol II transcription-activators and repressors Chapter 12, 342-377, Garvie, Darnell, Pandolfi Sess. 6 Ansari
7 Feb 11 Chromatin/histone modification Chapter 13, 380-415, Marmorstein Sess. 7 Wassarman
8 Feb 13 Chromatin remodeling Lusser, Iizuka, Rice Sess. 8 Wassarman
9 Feb 18 Current topics in transcriptional regulation-Dosage compensation Akhtar, Brockdorff Sess. 9 Wassarman
10 Feb 20 Exam 1 Exam 1 answers   Wassarman/Ansari
11 Feb 25 RNA processing-pre-mRNA splicing Chapter 14, 418-450 Sess. 11 Wassarman
12 Feb 27 RNA processing-capping and polyadenylation Chapter 15, 462-494 Sess. 12 Wassarman
13 Mar 3 RNA processing-rRNA, tRNA, trans-splicing, and RNA editing

Chapter 14, 450-458
Chapter 16, 497-520

Sess. 13 Wassarman
14 Mar 5 Post-transcriptional regulation of mRNA-stability and transport Wagner, Wilusz, Bentley Sess. 14 Wassarman
15
Mar 10
Current topics in the regulation of gene expression by RNA-RNA interference (RNAi/siRNA) Chapter 14, 520-524
Hannon, Zamore
Sess. 15 Wassarman
16 Mar 12 Translation-initiation Chapter 17, 545-564 Sess. 16 Wassarman
  Mar13-21 Spring recess      
17 Mar 24 Translation-elongation and termination Chapter 18, 567-603 Sess. 17 Wassarman
18 Mar 26 Ribosomes-structural considerations Chapter 19, 606-625
Noller web site,
http://rna.ucsc.edu/rnacenter/
Sess. 18 Wassarman
19 Mar 31 tRNA-regulatory and structural considerations Chapter 19, 626-639 Sess. 19 Ansari
20 Apr 2 Current topics in translational regulation-IRES Pdfs Sess. 20 Seiser
21 Apr 7 Exam 2 Exam 2 answers   Wassarman/Ansari
22 Apr 9 DNA replication-basic mechanism Chapter 20, 642-667 Sess. 22 Ansari
23 Apr 14 DNA replication-initiation, elongation, and termination Chapter 21, 684-708 Sess. 23 Ansari
24 Apr 16 Centromeres and telomeres Chapter 21, 708-716 Sess. 24 Ansari
25 Apr 21 Current topics in DNA metabolism-origin recognition complex (ORC) Stillman, Bell Sess. 25 Ansari
26 Apr 23 DNA damage and repair Chapter 20, 668-676 Sess. 26 Ansari
27 Apr 28 Homologous recombination Chapter 22, 717-745 Sess. 27 Ansari
28 Apr 30 Site-specific recombination Chapter 23, 747-767 Sess. 28 Ansari
29 May 5 Genomes and applications of genomics Chapter 24, 784-814 Sess. 29 Ansari
30 May 7 Topics that integrate multiple mechanisms in eukaryotic molecular biology-NF-kB Pdfs Sess. 30 Miyamoto
31 May 9-15 Exam 3 Exam 3 answers   Ansari