UW-Madison Department of Dairy Science

864 Animal Sciences Building
1675 Observatory Drive
Madison, WI 53706-1284
Phone: (608) 263-9867, (608) 263-3308
Fax: (608) 263-9412
lgsheffi@wisc.edu

Professor Sheffield's area of expertise is mammary gland biology and endocrinology. He teaches undergraduate classes in Physiology of Lactation and graduate classes in Lactation Biology.

Dr. Sheffield's research program is focused on understanding the mechanisms controlling mammary gland development and differentiation. Research projects include the mechanisms of hormone action on mammary tissue, cross talk among hormone signaling pathways and the molecular regulation of growth factor production in mammary tissue. Species studied include bovine, human and rodents. Model systems include whole animal studies, cell culture models and cell-free biochemical systems. Approaches are generally at a biochemical and molecular level.

Students in the Sheffield laboratory train in Dairy Science or in interdisciplinary training programs in Endocrinology-Reproductive Physiology or Environmental Toxiology. Prospective students should have a solid background in basic sciences, including biology, chemistry and biochemistry.

Representative Publications:

Vanderboom, R.J. and L.G. Sheffield. 1993. Estrogen enhances epidermal growth factor induced DNA synthesis in mammary epithelial cells. J. Cell. Physiol. 156:367-372.

Fenton, S.E. and L.G. Sheffield. 1993. Prolactin inhibits epidermal growth factor (EGF)-stimulated signaling events in mouse mammary epithelial cells by altering EGF receptor function. Mol. Biol. Cel 4:773-780.

Fenton, S.E. and L.G. Sheffield. 1994. Control of mammary epithelial cell DNA synthesis by epidermal growth factor, cholera toxin, and IGF-1: Specific inhibitory effect of prolactin on EGF-stimulated cell growth. Exp. Cell Res. 210:102-106.

Johnson, J.L., S.E. Fenton and L.G. Sheffield. 1996. Prolactin inhibits epidermal growth factor-induced ras-MAPK signaling in mammary epithelial cells. J. Biol. Chem. 271:21574-21578.

Fenton, S.E. and L.G. Sheffield. 1997. Prolactin inhibits EGF-induced DNA synthesis in mammary epithlium via early signaling mechanisms: Possible involvement of protein kinase C. Exp. Cell Res. 236:285-293.

Sorensen, P. and L.G. Sheffield. 1997. Involvement of c-src in beta-casein expression by mammary epithelial cells. Biochem. Biophys. Res. Commun. 241:710-713.

Quijano, V.J., Jr. and L.G. Sheffield. 1997. Prolactin decreases EGF - induced EGF receptor tyrosine phosphorylation via a phosphorylation-dependent mechanism. J. Biol. Chem. 273:1200-1207.

Fang, Y. and L.G. Sheffield. 1998. Regulation of EGF expression in mammary epithelial cells by a YY1-like element. J. Mo. Endocrinol. 20:337-344.

Sheffield, L.G. 1998. Hormonal regulation of epidermal growth factor receptor content and signaling in bovine mammary tissue. Endocrinology. 139:4568-4575.